PandaPure® revolutionizes purification using programmable, easy-to-grow organelles, instead of traditional solid supports in chromatography or other affinity methods.
— finally, your workflow can become simple, effective, and scalable.
Example: PandaPure Protein System for bacterial E. coli
Preparing PandaPure Organelle plasmid to express synthetic organelle (up), and cloning the target gene into the cognate PandaPure Expression Vector (bottom). Co-transforming two plasmids into a expression host, e.g., E. coli BL21(DE3).
Induce co-expression of organelles and targets with respective inducers (e.g., aTc and IPTG), or via auto-induction medium.
Open the cell structures by disrupting cell membrane and/or cell walls, to isolate organelles enriched with target proteins. If necessary for higher purity, organelles are further washed with proper buffers.
React the organelles with PandaPure® Protein Reagent, to mediate tag cleavages. Then recover the tag-less proteins in a simple centrifugation and/or filtration.
Explore different models to bring PandaPure into your facility.
Includes:
Core plasmids, at 5 μg each
Essential starter supply, at 50 mL *
License for research-use only
Includes:
High-quantity plasmids, from 10 μg
Key reagents, from 100 mL
Premium labware & consumables
License for research-use only
Includes:
Full access to plasmids
Bulk pricing for reagents
Advanced construct services
Licenses for commercial uses§
For our seasoned users, enhance your PandaPure journey with our top-notch components and services.
Discover how our biological programming transforms biotechnology into a programmable, efficient, and accessible technology.
PandaPure uses engineered biological compartments (synthetic organelles), often formed via Liquid-Liquid Phase Separation (LLPS). These DNA-programmed structures replace traditional purification media, offering novel functions beyond native cellular limits.
PandaPure replaces expensive, complex chromatography equipment (like FPLC) with programmable biological compartments working autonomously in your sample. Benefits include significantly reduced hands-on time and typically improved yields.
Current PandaPure Protein Expression and Purification kits are optimized for producing tag-free recombinant proteins from E. coli. Kits for other targets are coming soon. Follow us on social media or subscribe to our newsletter for updates and early access!
We have validated a wide range of methods, from sonication and mild detergents. You can also purchase optimized Lysis Reagent from us through Hi Panda kits.
No special equipment needed – just standard lab items like centrifuges, pipettes, and incubators/shakers. PandaPure eliminates the need for costly FPLC systems and columns.
Yes, PandaPure can enable high-throughput protocols using deep-well plates, liquid handling automation, and standard plate centrifuges (e.g., Eppendorf® 5804/5810).
Explore more about PandaPure® and synthetic organelle technologies.
Explore our innovations in designing and engineering synthetic RNA molecules for spatial organization, from synthetic scaffolding (Delebecque CJ, et al. Science 2011) to the first liquid-liquid phase separation system established in bacteria by engineered RNA molecules (Guo H, et al. Cell 2022), the foundation of PandaPure technologies.
Discover the power of synthetic organelles and how they can revolutionize biotechnology.
Download detailed step-by-step protocols for using PandaPure kits and optimizing your workflow.
