PandaPure - Effortless protein expression and purification

PandaPure

Cell-program assay to streamline protein expression and purification.
0 Column, 0 Protease, More Productive

Get the highlights.

PandaPure® offers you next-level experiences of protein research, by eliminating the need for traditional chromatography, buffer exchange and protease treatments, and improving expression of many difficult proteins. Now, you can obtain tag-free proteins with the fewest operations, reagents and the least effort.

Purification

Easier. Faster. Simpler.

Cells, not human, doing most of the works

Instead of human doing all the work, cells are programmed to purify proteins by themselves.

Expression

Next-level Performance

More proteins, more yields, more success

More proteins and more types of proteins can be expressed under PandaPure system

Efficiency

Recover up to 70% yield
‍of expressed target proteins.

Purity

Get >95%-purity
native proteins < 1 day.

Throughput

96 in Parallel.

Suitable for multi-well plates and automation

PandaPure is suitable with either manual high-throughput production, or automatic workstations.

Scalability

Scaling. Up and down.

From μL,
To liters.

PandaPure system can be scaled down to small volumes, and potentially up to very large volume of fermentation.

Scope of applications

Eukaryotic-like bacteria is more versatile, and more productive.

PandaPure brings traditional hosts like E. coli ¹ to the next level by synthesizing eukaryotic structures. Leveraging synthetic organelles, PandaPure creates a distinct environment for protein folding, maturation, and storage. This broadens the range of applicable proteins, also often results in higher production yields. See cases of PandaPured proteins, and join us to explore the new limits:

Superfolder GFP

PandaPure successfully produces GFP at high yield and purity.

DpnI, restriction enzyme

PandaPure successfully produces DpnI at high yield and purity.

Proinsulin, human insulin precursor

PandaPure successfully produces proinsulin at high yield and purity.

Getting started.

PandaPure requires engineered DNAs to program your bacterial hosts, and reagents to operate the downstream processing workflow. Here we offer predefined Kits and additional Construct Service, with the flexibility to order individual plasmids and reagents. Place an order >>

PandaPure®️ Kit

Predefined kits
for common applications

from lab-scale production
to high-throughput screening
Order now

PandaPure®️ Construct Service

Build your strains for PandaPure effortlessly.

Get ready-to-use DNA at a competitive price from US$0.1/bp²
Request services

PandaPure®️ Plasmids

Genetic elements that make cells purify proteins

Plasmids for synthetic organelles,
expression vectors, and for controls.
Read more

PandaPure®️ Protein Reagent

All reactions in one reagent,
including tag removal and protein recovery.

from $253.00

PandaPure®️ Lysis Reagent

Easy-to-use, gentle lysis
for bacteria hosts.

from $135.00

For commercial uses

MTA & licensing

Industrial users can license from us to exploit PandaPure commercially.
‍Highlight: Startup Plan

Take a closer look.

Cells are isolating your proteins.

Now, protein purification is

Easier than ever.

PandaPure is designed to minimize the complexity of protein purification, extracting pure proteins with only one step of pipetting and centrifugation. The hands-on workload becomes negligible. Now, anyone who had basic molecular biology training can apply protein purification in their research.

Save up to $300 per 1mg protein³

Up to 10x cheaper

PandaPure offers higher yield, higher purity, tag removal, high throughput enabling and more, only at the cost of simplistic his-tag affinity purification.

Sciences behind PandaPure

Synthetic sorting

PandaPure® Protein Constructs encode proteins of interest (POI), and also synthetic organelles with cognate receptors. Thus, synthetic organelles can selectively sort, purify, and store proteins of interest.

Protein recovery

In a single reaction under mild conditions

PandaPure® Protein Reagent induces self-cleavage of signal tags, thus enables the extraction of tag-free proteins in a single reaction.

How to cite PandaPure in your article

We thank you for applying PandaPure in your research. Please cite PandaPure and include Ailurus in your future publications. Proper citation is important for the scientific community to better understand, reproduce and promote your research. It also helps acknowledge the development and refinement of the method, supporting further advancement in research.

For your Materials & Methods section:

Recombinant proteins were expressed using PandaPure constructs and reagents (Ailurus, London, United Kingdom), following the workflow previously described [Reference number].

For your References section:

For PandaPure:

‍Guo, Haotian, et al. "Synthetic organelles enable protein purification in a single operation." bioRxiv (2024): 2024-05.

For synthetic organelles:

Guo, Haotian, et al. "Spatial engineering of E. coli with addressable phase-separated RNAs." Cell 185.20 (2022): 3823-3837.‍‍

"Traditional E. coli" refers to commonly-used, commercially-available bacteria strains such as BL21(DE3), and expression vectors such as pET-28a(+). For some proteins, other strain engineering methods to modify genome and plasmid sequences may lead to optimization in protein expression.
Gene synthesis prices are considered "competitive" based on publicly listed pricing from DNA and gene synthesis suppliers as of Dec 15, 2023. Turnaround times may vary due to complexity of the order, and shipments.
Savings based on publicly listed pricing as of Dec 15, 2023 of products from Thermo Fisher Scientific and Cytiva, in order to process 1 mg protein. Loss of proteins in each step is not counted. The exact saving can vary according to the differences among experiments, and may be even higher than what we estimate here.